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HPLC chromatogram of 11 nicotinamide metabolites (sample concentration; 250 µM, injection volume; 1.0 µL) obtained using a COSMOSIL 3PBr column (3.0 mm I.D. × 150 mm, particle size; 3 µm) with a flow rate of 0.4 mL/min at 40 ºC. UV detection was performed at 260 nm. * Peak of impurities derived from NAD + .

Journal: MethodsX

Article Title: Separation of highly hydrophilic nicotinamide metabolites using a COSMOSIL PBr column

doi: 10.1016/j.mex.2023.102061

Figure Lengend Snippet: HPLC chromatogram of 11 nicotinamide metabolites (sample concentration; 250 µM, injection volume; 1.0 µL) obtained using a COSMOSIL 3PBr column (3.0 mm I.D. × 150 mm, particle size; 3 µm) with a flow rate of 0.4 mL/min at 40 ºC. UV detection was performed at 260 nm. * Peak of impurities derived from NAD + .

Article Snippet: Adenosine triphosphate (ATP; Cat. No. 01072–11), nicotinic acid (NA; Cat. No. 24326–52), nicotinamide (NAM; Cat. No. 24317–72), inosinic acid (IMP; Cat. No. 06400–51), inosine (Cat. No. 07139–42), nicotinamide adenine dinucleotide in oxidized form (NAD + ; Cat. No. 24338–86), nicotinamide adenine dinucleotide in reduced form (NADH; Cat. No. 24335–61), methanol (HPLC grade, Cat. No. 21929–23), chloroform (HPLC grade, Cat. No. 08426–13), and ammonium formate (guaranteed reagent, Cat. No. 02509–55) were obtained from Nacalai Tesque Inc. (Kyoto, Japan).

Techniques: Concentration Assay, Injection, Derivative Assay

(a) HPLC traces of various concentrations of nicotinamide mononucleotide (NMN), nicotinamide (NAM), and nicotinamide adenine dinucleotide (NAD + ) separated using a COSMOSIL 3PBr column (3.0 mm I.D. × 150 mm, particle size; 3 µm) with a flow rate of 0.4 mL/min at 40 ºC. UV detection was performed at 260 nm. * Peak of impurities derived from NAD + . (b) Calibration curves of NMN, NAM, and NAD + .

Journal: MethodsX

Article Title: Separation of highly hydrophilic nicotinamide metabolites using a COSMOSIL PBr column

doi: 10.1016/j.mex.2023.102061

Figure Lengend Snippet: (a) HPLC traces of various concentrations of nicotinamide mononucleotide (NMN), nicotinamide (NAM), and nicotinamide adenine dinucleotide (NAD + ) separated using a COSMOSIL 3PBr column (3.0 mm I.D. × 150 mm, particle size; 3 µm) with a flow rate of 0.4 mL/min at 40 ºC. UV detection was performed at 260 nm. * Peak of impurities derived from NAD + . (b) Calibration curves of NMN, NAM, and NAD + .

Article Snippet: Adenosine triphosphate (ATP; Cat. No. 01072–11), nicotinic acid (NA; Cat. No. 24326–52), nicotinamide (NAM; Cat. No. 24317–72), inosinic acid (IMP; Cat. No. 06400–51), inosine (Cat. No. 07139–42), nicotinamide adenine dinucleotide in oxidized form (NAD + ; Cat. No. 24338–86), nicotinamide adenine dinucleotide in reduced form (NADH; Cat. No. 24335–61), methanol (HPLC grade, Cat. No. 21929–23), chloroform (HPLC grade, Cat. No. 08426–13), and ammonium formate (guaranteed reagent, Cat. No. 02509–55) were obtained from Nacalai Tesque Inc. (Kyoto, Japan).

Techniques: Derivative Assay

LC–MS chromatograms of 11 nicotinamide metabolites separated using a COSMOSIL 3PBr column (3.0 mm I.D. × 150 mm, particle size; 3 µm) with a flow rate of 0.4 mL/min at 40 ºC.

Journal: MethodsX

Article Title: Separation of highly hydrophilic nicotinamide metabolites using a COSMOSIL PBr column

doi: 10.1016/j.mex.2023.102061

Figure Lengend Snippet: LC–MS chromatograms of 11 nicotinamide metabolites separated using a COSMOSIL 3PBr column (3.0 mm I.D. × 150 mm, particle size; 3 µm) with a flow rate of 0.4 mL/min at 40 ºC.

Article Snippet: Adenosine triphosphate (ATP; Cat. No. 01072–11), nicotinic acid (NA; Cat. No. 24326–52), nicotinamide (NAM; Cat. No. 24317–72), inosinic acid (IMP; Cat. No. 06400–51), inosine (Cat. No. 07139–42), nicotinamide adenine dinucleotide in oxidized form (NAD + ; Cat. No. 24338–86), nicotinamide adenine dinucleotide in reduced form (NADH; Cat. No. 24335–61), methanol (HPLC grade, Cat. No. 21929–23), chloroform (HPLC grade, Cat. No. 08426–13), and ammonium formate (guaranteed reagent, Cat. No. 02509–55) were obtained from Nacalai Tesque Inc. (Kyoto, Japan).

Techniques: Liquid Chromatography with Mass Spectroscopy

LC–MS chromatograms of nicotinamide metabolites in tomato separated using a COSMOSIL 3PBr (3.0 mm I.D. × 150 mm, particle size; 3 µm) with a flow rate of 0.4 mL/min at 40 ºC. * Other compounds than NMN.

Journal: MethodsX

Article Title: Separation of highly hydrophilic nicotinamide metabolites using a COSMOSIL PBr column

doi: 10.1016/j.mex.2023.102061

Figure Lengend Snippet: LC–MS chromatograms of nicotinamide metabolites in tomato separated using a COSMOSIL 3PBr (3.0 mm I.D. × 150 mm, particle size; 3 µm) with a flow rate of 0.4 mL/min at 40 ºC. * Other compounds than NMN.

Article Snippet: Adenosine triphosphate (ATP; Cat. No. 01072–11), nicotinic acid (NA; Cat. No. 24326–52), nicotinamide (NAM; Cat. No. 24317–72), inosinic acid (IMP; Cat. No. 06400–51), inosine (Cat. No. 07139–42), nicotinamide adenine dinucleotide in oxidized form (NAD + ; Cat. No. 24338–86), nicotinamide adenine dinucleotide in reduced form (NADH; Cat. No. 24335–61), methanol (HPLC grade, Cat. No. 21929–23), chloroform (HPLC grade, Cat. No. 08426–13), and ammonium formate (guaranteed reagent, Cat. No. 02509–55) were obtained from Nacalai Tesque Inc. (Kyoto, Japan).

Techniques: Liquid Chromatography with Mass Spectroscopy

Journal: MethodsX

Article Title: Separation of highly hydrophilic nicotinamide metabolites using a COSMOSIL PBr column

doi: 10.1016/j.mex.2023.102061

Figure Lengend Snippet:

Article Snippet: Adenosine triphosphate (ATP; Cat. No. 01072–11), nicotinic acid (NA; Cat. No. 24326–52), nicotinamide (NAM; Cat. No. 24317–72), inosinic acid (IMP; Cat. No. 06400–51), inosine (Cat. No. 07139–42), nicotinamide adenine dinucleotide in oxidized form (NAD + ; Cat. No. 24338–86), nicotinamide adenine dinucleotide in reduced form (NADH; Cat. No. 24335–61), methanol (HPLC grade, Cat. No. 21929–23), chloroform (HPLC grade, Cat. No. 08426–13), and ammonium formate (guaranteed reagent, Cat. No. 02509–55) were obtained from Nacalai Tesque Inc. (Kyoto, Japan).

Techniques: Modification, Mass Spectrometry, Chromatography